17+ a modular cloning toolkit for genome editing in plants
We believe the presented CRISPRCas toolkit is a great resource that will contribute towards wider adoption of the CRISPRCas genome editing technology and. The modular cloning MoClo based on the Golden Gate GG method has enabled development of cloning systems with standardised genetic parts eg.
A Modular Cloning Toolkit For Genome Editing In Cereals Springerlink
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. A modular cloning toolkit for genome editing in plants bioRxiv. The three panels shown come from different parts of the same DNA gel Additional file 4. Is a cloning method that is able to efficiently assemble multiple expression units and has been used.
An expanded cloning toolkit that contains 103 modules encoding a variety of CRISPRCas-based nucleases and their corresponding guide RNA backbones is presented that contains a set of. NTC is the no template control - A modular cloning toolkit for genome. M is the DNA marker.
PDF Background CRISPRCas has recently become a widely used genome editing tool in various organisms including plants.
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